What we do

Signum Biosciences is a private biotech company founded in 2003 from technologies developed by Dr. Jeffry Stock at Princeton University.  With over 15 years of developing multiple ingredients and botanical extracts from discovery to fully formulated skin-care products that are on the market, Signum possesses the ability to streamline your research and product development.  We specialize in producing customized claim support and dermatological efficacy testing to provide clients with robust scientific data needed to substantiate product claims and/or compare head to head versus competitors. We work with clients ranging from multi-national companies to virtual biotech startups, all projects are welcomed.  We have highly trained scientists who can tailor a research plan to fit your requirements be it efficacy, safety, regulatory or scientific marketing. Whether you are early in discovery phase, development, clinical stage, or ready to commercialize your products, Signum can provide the following services to aid you on your product development path.

 

  • Standard assays to determine activity profile
  • Safety and regulatory support
  • Customized screening
  • Topical formulation development, stability and dermal penetration studies
  • Scientific marketing and peer-reviewed publishing of results
  • Botanical extraction, characterization and manufacturing
  • Compound synthesis and manufacturing

           

Signum is the solution to optimize your R&D screening, product development and customized research at a reduced cost compared to larger CRO’s. We provide superior service to differentiate your product, so contact us today. Our discussions will provide the scientific excellence and quality your product deserves.

 

For a full list of services we offer, download the PDF below.

Downoad Full Catalog PDF

 

Standard Assays

Signum has a host of standard assays that can be performed to test your ingredient, compound, or formulated product.  With assay systems in areas of inflammation, aging, pollutant toxicity, anti-microbial, anti-oxidant, skin whitening and tanning, wound healing, and metabolism, Signum is able to provide a full-spectrum of research methods to suite your needs.  We possess in vitro capabilities for molecular techniques including enzymatic evaluation, gene and protein expression, cell line expansion, intracellular pathway analysis using colorimetric, fluorescent or histology-based methods.

 

Inflammation

Catalog #

Model

Stimulus/Inducer

Endpoint

Method

 Reference

SKI-001

Human Epidermal Keratinocytes (NHEKs) or Human Reconstructed Epidermis (EpiDerm™)

TPA/PMA

Chemical irritation

Cytokine ELISA

Glucocorticoid

SKI-002

UVB

Photodamage

SKI-003

Sunburn Cells

Vitamin C

SKI-004

DNA pyrimidine dimers

SKI-005

Lipid Peroxides

SKI-006

Peripheral Blood Mononuclear Cells (PBMC)

anti-CD3/CD28

T-cell activation

Th2/Th17-marker ELISA

TGF-beta

SKI-007

Human Dermal Microvascular Endothelial Cells (HDMECs)

ATP-gS

ATP inflammation

MTS Viability, Cytokine ELISA

Glucocorticoid

SKI-008

anti-microbial peptide (LL-37)

Cathelicidin

SKI-009

Nickel sulfate

Nickel Allergy

SKI-010

Human Epidermal Keratinocytes (NHEKs)

C. acnes, PGN

IL-8

Dexamethasone

SKI-011

Human Reconstructed Cornea (EpiOcular™)

Dry Eye

Viability, MMP9

 

Aging

Catalog #

Model

Stimulus/Inducer

Endpoint

Method

 Reference

SKA-001

Human Epidermal Keratinocytes (NHEKs)

Cell Confluence

Epidermal Differentiation

Keratin-1, Filaggrin, Loricrin

Adapalene

SKA-002

Blue Light

Photoaging

Viability, HAS2

Vitamin C

SKA-003

Human Adult Dermal Fibroblasts (HDFs) or Human Reconstructed Epidermis (EpiDermFT™)

Basal

Extra cellular matrix expression

MMPs, Collagen, Elastin,

Vitamin C, TGF-beta

Fibronectin,

SKA-004

UVA

Photoaging

Laminin

SKA-004

H2O2

Senescence

Histology

 

Pollutant Toxicity

Catalog #

Model

Stimulus/Inducer

Endpoint

Method

 Reference

TOX-001

Human Epidermal Keratinocytes (NHEKs)

Urban Dust Particulate

Air Pollution

MTS Viability, Cytokine ELISA

Dexamethasone

TOX-002

Human Dermal Microvascular Endothelial Cells (HDMECs)

Cadmium

Metal Pollution

TOX-003

Nickel

 

Anti-Microbial

Catalog #

Model

Stimulus/Inducer

Endpoint

Method

 Reference

AMIC-001

Cutibacterium acnes

Minimum inhibitory Concentration

Anti-bacterial

Culture Turbidity, Colony counting

Doxycycline

AMIC-002

Staphylococcus epidermidis

AMIC-003

Staphylococcus aureus

AMIC-004

Streptococcus pyogenes

AMIC-005

Escherichia coli

AMIC-006

Pseudomonas aeruginosa

Gentamycin

AMIC-007

Candida albicans

TYMC

Anti-fungal

Colony counting

Fluconazole

AMIC-008

Aspergillus brasiliensis

Benzalkonium Chloride

 

Anti-Oxidant

Catalog #

Model

Stimulus/Inducer

Endpoint

Method

 Reference

AOX-001

Cell-free antioxidant

H2O2

ROS scavenging

ABTS

Vitamin C, Ferulic Acid

AOX-002

Human Adult Dermal Fibroblasts (HDFs)

Intracellular ROS

DCF-DA

AOX-003

Low Density Lipoproteins

Fe2SO4

Lipid Peroxidation

Hydroperoxide

AOX-004

Microsomes

NADPH/ADP/Fe3+

Membrane MDA

TBARS

AOX-005

Human Neutrophils HL-60

fMLP/PMA

Cell Oxidative Burst

Colorimetric

Vitamin E

 

Skin Whitening

Catalog #

Model

Stimulus/Inducer

Endpoint

Method

 Reference

SKW-001

Cell-free Tyrosinase

Basal

Tyrosinase inhibition

Colorimetric

Kojic Acid

SKW-002

Human Reconstructed Epidermis (MelanoDerm™)

Melanin content

SKW-003

Epidermal Melanocytes

 

Skin Tanning

Catalog #

Model

Stimulus/Inducer

Endpoint

Method

 Reference

SKT-001

Human Reconstructed Epidermis (MelanoDerm™)

Basal

Melanin content

Colorimetric

IBMX

SKT-002

Epidermal Melanocytes

 

Wound Healing

Catalog #

Model

Stimulus/Inducer

Endpoint

Method

 Reference

WH-001

Human Epidermal Keratinocytes (NHEKs)

in vitro wound (scratch assay)

Cell Migration

Histology

EGF

WH-002

Human Dermal Fibroblasts (HDFs)

FGF

 

Metabolism

Catalog #

Model

Stimulus/Inducer

Endpoint

Method

 Reference

MET-001

Human Epidermal Keratinocytes (NHEKs) or Human Reconstructed Epidermis (EpiDerm™)

Basal

Mitochondrial localization

Histology

EGF

 

Safety and Regulatory Support

Equally important to determining if your ingredient possesses beneficial skin properties is to ensure that it is safe when topically applied. We perform a battery of tests to ensure your ingredient possesses a safety profile adherent to industry standards and can provide regulatory support in getting your novel ingredient or extract registered with the Personal Care and Products Council for INCI designation.

Catalog #

Model

Stimulus/Inducer

Endpoint

Method

 Reference

SAF-001

OECD TG 439: Human Reconstructed Epidermis (EpiDerm™)

Basal

Skin Irritation

MTT

SDS

SAF-002

OECD TG 492: Human Reconstructed Cornea (EpiOcular™)

Basal

Ocular irritation

MTT

Methyl Acetate

SAF-003

OECD TG 432: Mouse BALB/3T3 Fibroblasts

UVA

Phototoxicity

Neutral

Chlorpromazine

Red Uptake

SAF-004

Human Reconstructed Skin (EpiDerm FT™)

UVA

Photodamage

MTT

Chlorpromazine

SAF-005

UVB

Sun Screen

SAF-006

Infrared-A

Vitamin C

SAF-007

OECD TG 428: Skin Absortion

Basal

Dermal Penetration

Franz Cell

Vehicle

 

Formulation

After determining the activity and safety profile of your ingredients, Signum can provide formulation services to move forward the development of your actives into a formulated product.  Signum has the capabilities to develop your ingredients into any desired formulation (i.e. cream, lotion, gel, toner, serum, etc.).  Along with formulation development, we offer related services in formulation including stability testing, preservative testing, and Franz cell dermal penetration studies.  Utilizing reconstructed 3D human skin tissues, your final formulated product can be applied topically and tested for efficacy in vitro and, if desired, compared head to head to competitor products to aid in claim substantiation and marketing.

 

Customized Screening

Standard dermatological tests, while effective in many respects, do not always provide the best path forward in characterizing the activity of your ingredient or product. Given our past experience in working to develop and commercialize cosmetics, botanical extracts, pharmaceuticals and nutraceuticals, we possess innovative capabilities in protocol design, assay development and claim support testing. With the support of our knowledgeable project management team, we can demonstrate the activity of your molecule or product by personalizing an experimental plan and assay system to produce exciting scientific data to deliver your study objectives and support your product’s brand goals.  

 

Other Services

Signum strives to offer comprehensive services to our clients and their ingredients or products no matter what stage of development you are in.  We have experience taking ingredients from discovery to market and can assist you with any and all steps along the way.  Listed below are some of the additional services that we offer.

  • Botanical Extraction
    • Signum has experience fractionating botanical extracts to identify the key ingredients driving activity and characterization. Once identified the extract can be scaled up, assessed for stability, studied analytically to set specifications and then moved into testing with several formulation prototypes
  • Scientific Marketing (manuscripts, posters, etc.)
    • Signum understands the importance of giving your product credibility and differentiation from competitors. We offer scientific writing services such as manuscripts or posters for your ingredient or product which can be submitted to present in scientific conferences or for peer-reviewed publication. 
  • Medicinal Chemistry and Scale Manufacturing
    • Given our expertise in Rx development and synthesizing novel compounds, we can assist your efforts in synthesizing compounds, making a series of derivatives or scaling up your current ingredient to kilo scale. Beyond that, our Florida facility can perform large scale manufacturing to 100+ kg once your product is on the market

 

Early development and precise strategy can reduce costs and accelerate timelines. Contact us to learn more about all our services and how Signum Biosciences can bring maximum benefit to your development program and product.